This kit is used for the quantitative determination of Luteinizing Hormone (LH) in human urine samples in vitro.
Human luteinizing hormone (LH) is a kind of glycoprotein hormones secreted by the pituitary gland, exists in human blood and urine, its role is to stimulate the release of the ovaries mature eggs. LH in the middle of the menstrual secretion, sharply form LH peak, from the basic level of 5 ~ 20 miu/ml rapidly rising to the peak of 25 ~ 200 miu/ml.Urine LH concentration is usually about 36 ~ 48 hours before ovulation suddenly jumped sharply, in 14 ~ 28 hours peak, peak about 14 to 28 hours after theca burst, mature eggs. LH within two days after ovulation back to the basic level, to enter the luteal phase, luteal phase usually lasts for 14 days. Then, the new follicles began to development, into the next menstrual cycle, so the cycle, until the pregnancy. Women in the LH peak within 1 ~ 3 days after the most fertile, thus, detection of LH in the urine levels can be used to predict ovulation time, can grasp the conception time effectively.
[Test principle]
This product uses the colloidal gold immune technology and the principle of double antibody sandwich method to qualitatively detect the LH presence level in female urine. A quality control line (line c) on that fib membrane in the reagent card is coated with goat anti-mouse IgG, a detection line (line t) is coat with an anti-LH monoclonal antibody -2, and the other end is coated with an anti-LH monoclonal antibody -1- colloidal gold complex. Dure detection, if LH is present in that urine sample, a complex is formed with the anti-LH monoclonal antibody -1 on colloidal gold and run on the membrane,