Myocardial infarction test kit OTK003

creatine kinaseserumplasma

This kit is used for the quantitative determination of Creatine Kinase Isoenzyme MB (CK-MB) in human serum, plasma or whole blood samples in vitro. There are four isozyme forms of Creatine Kinase (CK): muscle type (MM), brain type (BB), mixed type (MB) and mitochondrial type (MiMi), among which MB type is mainly present in myocardial cells. In myocardial infarction, creatine kinase increases within 6 hours of onset, peaks at 24 hours, and returns to normal within 3~4 days. Among them, Creatine Kinase Isoenzyme MB MB has high diagnostic specificity, so it has become one of the markers of myocardial infarction. [Test principle] This test kit uses fluorescence immunochromatography technology to quantitatively determine the content of Creatine Kinase Isoenzyme MB (CK-MB) in serum, plasma or whole blood samples by double antibody sandwich method. After the sample is added to the test kit, the CK-MB in the sample reacts with the fluorescently labeled CK-MB monoclonal antibody 1 to form a complex, which continues forward chromatography along the nitrocellulose membrane to the test line, and forms a complex binding with coated CK-MB monoclonal antibody on the test line. Under the action of the excitation light source, the content of Creatine Kinase Isoenzyme MB (CK-MB) in the sample is positively correlated with the fluorescent antibody signal, and the content of Creatine Kinase Isoenzyme MB (CK-MB) in the sample was calculated by fluorescence immunoanalyzer. Principle of the supporting instrument: The measuring system of the instrument scans the binding area between the marker and the analyte on the test kit after the reaction to obtain the optical signal.