The HbA1c test kit is applicable to the clinical determination of the content of human blood for glycosylated hemoglobin, and a specific reagent for the specific protein analyzer applies only to the clinical in vitro assisted diagnosis. Glycosylated hemoglobin is the formation of hemoglobin and a combination of sugars by non-enzymatic. The combination of processes is slow and relatively irreversible, and the persistence of the life of 120 days in the red blood cells is directly proportional to the concentration of the sugar in the synthesis rate of erythrocyte surroundings. Therefore, the percentage of glycosylated hemoglobin reflects the determination of the average blood sugar levels within 8-12weeks before testing.
Testing Principle of Hemoglobin A1c (HbA1c) Reagent Blood Test Kit
The HbA1c unit is the use of antigen-antibody reaction to the direct determination of HbA1c percentage of total Hb.
Samples of total Hb, HbA1c, and latex in the sample will be solidified by the same non-specific adsorption.
The latex-HbA1c-mouse anti-human HbA1c monoclonal antibody complexes formed when the HbA1c of specific monoclonal antibodies joined in.
This composite was agglutinating because of sheep anti-mouse lgG antibodies and will produce the phenomenon of light scattering.
Content is proportional to the intensity of scattered light and samples of HbA1c.
Specific protein immunoassay to measure the intensity of scattered light, and comparison with the standard curve of HbA1c percentage concentration to get HbA1c percentage of the total Hb content in the sample.