The Dengue IgG/IgM/NS1 Combo Rapid Test Device is a lateral flow chromatographic immunoassay for the qualitative detection of dengue IgG/ IgM and virus antigen (Dengue Ag) in human whole blood, serum or plasma.
PRINCIPLE
The Dengue IgG/IgM Rapid test is a lateral flow chromatographic immunoassay.The test cassette consists of: 1) a burgundy colored conjugate pad containing dengue recombinant envelope antigens conjugatedwith colloid gold (dengue conjugates) and rabbit IgG-gold conjugates,2) a nitrocellulose membrane strip containing two test bands (G and M bands) and a control band (C band).The G band is pre-coated with the antibody for the detection of IgG anti-dengue virus, M band is coated with antibody for the detection of IgM anti-dengue virus, and the C band is pre-coated with goat anti rabbit IgG. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. IgG anti-dengue virus if present in the specimen will bind to the dengue conjugates. The immunocomplex is then captured by the reagent coated on the G band, forming a burgundy colored G band, indicating a dengue virus IgG positive test result and suggesting a recent or repeat infection.
Dengue fever is a debilitating viral illness transmitted by mosquitoes, particularly the Aedes aegypti species. Early diagnosis and management are crucial in preventing severe complications and controlling the spread of the disease. The Dengue IgG/IgM/NS1 Combo Rapid test emerges as a valuable tool for efficient and accurate diagnosis, enabling prompt intervention and effective control measures.