Home use rapid test FIA VD

for antibodiesbloodimmunochromatographic

【INTENDED USE】 The VD Semi-Quantitative FIA test Cassette (FIA) is a rapid chromatographic immunoassay for the semi-quantitative detection of 25-hydroxy Vitamin D in whole bloodwhich is mainly used to evaluate the levels of vitamin D. 【PRINCIPLE】 The membrane of the test device is coated with the conjugate of anti-25-(OH)VD antibody on the test region and goat anti rabbit IgG antibody on the control region. Marker pad are coated by fluorescence marked anti 25-(OH)VD antibody and rabbit IgG in advance. When testing sample, 25-(OH)VD in sample combine with fluorescence marked anti 25-(OH)VD antibody, and form immune mixture. Under the action of the immunochromatography, the complex flow in the direction of absorbent paper, when complex passed the test region, complex fluorescent marker will be combined with anti-25-(OH)VD antibody on the membrane. If the concentration of 25-(OH)VD in specimen is higher than 30ng/mL only T1 line can be observed with the reader. And if the concentration is higher than 60ng/mL T2 line and T1 line can be observed together. The C line will always be observed in any concentration of the specimen. When the cassette inserted into the reader the fluorescence marked line will be seen as red line.