Serum reagent kit IB47600

plasmaagarose gelblood sample

Sample Size: Scalable for blood, buffy coat, serum, plasma cultured cells and tissue Operation time: up to 60 minutes Can simultaneously extract DNA – RNA and Protein from the same sample. IBI Isolate is a phenol, chloroform, and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA, and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells, and tissue. Sample:Adherent Cultured Cells, Suspension Cultured Cells, Body fluids including, Blood, Buffy Coat, Serum, and plasma. Format:Phenol, chloroform, and guanidine isothiocyanate based scalable solution. Extract simultaneous RNA-DNA and protein from the same sample using phase separation. The extracted RNA can be used directly in a variety of downstream applications such as cDNA library construction, cloning, RT-PCR (Endpoint), real-time PCR, nuclease protection assays, and northern blotting. Quality Control IBI Isolate is tested on a lot-to-lot basis. RNA from a 1 ml human blood sample is extracted using IBI Isolate. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel. How does the IBI Isolate DNA/RNA Reagent Sample Kit work?This kit uses a phenol-chloroform and guanidine isothiocyanate solution to simultaneously extract DNA, RNA and protein from a variety of samples. Once the sample is lysed, aqueous layers will form in the tube. The first, clear layer contains RNA, the second, white layer contains DNA & protein, the third, red layer contains organics, DNA and protein.