Solution reagent X-GAL

for cell culturefor molecular biologyliquid

Used as a histochemical substrate for β-galactosidase which allows the detection of lac gene expression X-GAL is a histochemical substrate for β-galactosidase which allows the detection of lac gene expression. To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20 mg/ml solution. Use either a glass or polypropylene tube. Add 4 ml of this solution per liter of culture media. Molecular Formula C14H15BrClNO6 Molecular Weight 408.64 Purity Min. 99% Solubility (2%, in N,N’ DMF) Clear and colorless Molecular Biology Specifications Optical rotation (C=1) - Pass Recommended Use A histochemical substrate for β-galactosidase, allowing the detection of lac gene expression. To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20 mg/ml solution. Use either a glass or polypropylene tube. Add 4 ml of this solution per liter of culture media. The tube containing the solution should be wrapped in aluminum foil to prevent damage by light and should be stored at –20°C. (It is not necessary to sterilize X-GAL solutions by filtration.) What can X-Gal be dissolved in or re-suspended in? Dimethylformamide (DMF) and Dimethyl sulfoxide (DMSO) How long can this be out of the refrigerator? Not more than 4-6 hours. *If solution turns pink, discard.