Illumina DNA PCR-Free Library Prep offers flexibility for many whole-genome sequencing applications.
Fast, automatable workflow, with ~1.5 hours total time
Flexibility to accommodate variations in sample type, DNA input amount, and application
Optimized library prep performance, generating reliable results
Save Time and Resources
The Illumina DNA PCR-Free Library Prep Kit uses a fast, user-friendly workflow. On-Bead Tagmentation chemistry reduces total library prep time to ~1.5 hours, from DNA extraction to library normalization.
Simplify Lab Operations
The Illumina DNA PCR-Free workflow supports a broad DNA input range (25 ng to 300 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots.
You can use liquid-handling robots to automate workflows for minimal touch points and significant time savings.
Obtain Reliable Results
While accommodating various study requirements, the Illumina DNA PCR-Free workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, delivering accurate base calling and variant identification for a broad range of DNA input amounts.
Access Flexible Throughput Options
The Illumina - DNA/RNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing that enables accurate assignment of reads and efficient use of the flow cell. These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.