Solution reagent kit AmpliSeq™ BRCA

for researchfor PCRfor DNA sequencing

The AmpliSeq for Illumina BRCA Panel is a targeted resequencing assay for research of somatic and germline variants across BRCA1 and BRCA2 genes. Key features include: Relevant Gene Content Target all exonic regions and flanking intronic sequences of BRCA1 and BRCA2 Fast, Streamlined Workflow Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples Accurate Data Detect somatic mutations as low as 5% variant allele frequency using local or cloud based analysis The BRCA Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis. BRCA1 and BRCA2 are human tumor suppressor genes that, when carrying specific mutations, have been implicated in an increased risk for breast and ovarian cancers. This ready-to-use panel saves researchers the time and effort of identifying targets, designing primers, and optimizing panels. Specifications Assay time - 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) Cancer type - Solid tumor Content specifications - Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 Description - Germline and somatic analysis studies of BRCA1 and BRCA2. Hands-on time - <1.5 hr Input quantity - 1–100 ng (10 ng recommended per pool) Instruments - MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System Method - Targeted DNA sequencing, Amplicon sequencing Multiplexing - 96 dual index combinations