Solution reagent kit AmpliSeq™

for researchfor PCRblood sample

The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database. Key features include: Relevant Gene Content Target ~2800 COSMIC mutations from 50 oncogenes and tumor suppressor genes Fast, Streamlined Workflow Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) samples Accurate Data Detect somatic mutations down to 5% variant allele frequency using local or cloud-based analysis The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis. This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. Specifications Assay time - 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) Cancer type - Pan-cancer, Solid tumor Content specifications - Hotspot regions of 50 genes with known associations to cancer Description - Somatic analysis research into hotspot regions of 50 cancer-related genes. Hands-on time - < 1.5 hr Input quantity - 1–100 ng (10 ng recommended per pool)