Solution reagent kit AmpliSeq™ Focus

for PCRblood samplefor DNA sequencing

The AmpliSeq for Illumina Focus Panel is a targeted resequencing assay for biomarker analysis of 52 genes with known relevance to solid tumors. Using the Focus Panel, researchers can analyze both DNA and RNA concurrently. Key features include: Relevant Gene Content Target biomarkers across 52 genes relevant to solid tumors Fast, Streamlined Workflow Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples Accurate Data Detect somatic mutations down to 5% frequency using local or cloud-based analysis The Focus Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis. This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. Specifications Assay time - 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) Cancer type - Solid tumor Description - Somatic analysis research on 52 genes associated with solid tumor cancers. Hands-on time - < 1.5 hr Input quantity - 1–100 ng (10 ng recommended per pool) Number of amplicons - 553 total. 2 pools. (DNA Pool: 269 amplicons. RNA Pool: 284 amplicons.) Specialized sample types - FFPE tissue Variant class - Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs)