Solution reagent kit AmpliSeq™

for histologyfor researchfor PCR

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel is a highly multiplexed targeted resequencing panel to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. Use a single pool of multiplex PCR primers, library reagents, and sample barcodes, to generate libraries from RNA extracted from whole blood, fresh-frozen tissue, or FACS-sorted cells. Key features include: High-Value Content Obtain comprehensive coverage of TCR beta chain sequences Fast, Streamlined Workflow Prepare sequencing-ready libraries in a single day from whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS) cells Low input requirement Generate high-quality sequencing libraries from as little as 10 ng of input RNA The Immune Repertoire Plus, TCR beta Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis. This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. Specifications Assay time - 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) Content specifications - RNA evaluation of TCRβ chain rearrangements, including CDR1, CDR2, and CDR3 (with up to 400 bp read-length amplicons) Hands-on time - < 1.5 hr Multiplexing - 96 dual index combinations