Solution reagent kit AmpliSeq™

for PCRblood sampleNGS

The AmpliSeq for Illumina Immune Response Panel is a targeted resequencing research assay for quantitating expression of cancer biomarkers in 395 genes involved in tumor–immune system interactions. Panel features Relevant Gene Content - Targeted expression of 395 cancer biomarkers indicative of immunotherapy response Fast, Streamlined Workflow - Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality RNA or 10 ng of formalin-fixed, paraffin-embedded (FFPE) RNA input samples Accurate Data - Detect expression of genes involved in tumor-immune interactions The Immune Response Panel is part of an integrated RNA-to-annotated variant solution that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis. Specifications Assay time - 6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) Content specifications - 395 genes associated with immune response Hands-on time - < 1.5 hr Input quantity - 1–100 ng (10 ng recommended per pool) Method - Amplicon sequencing, Targeted RNA sequencing Multiplexing - 96 dual index combinations Nucleic acid type - RNA Number of amplicons - 398 total (1 pool) Specialized sample types - FFPE tissue Variant class - Transcript variants