Multi-sample needles for single use is to be integrated used with needle holder. Unique double-bevel technique on the needle stick is specially designed for venous blood collection. The smooth cutting edge and silicone lubricated needlepoint reduce frictional resistance, which brings great experience of venipuncture for both medical personnel and patients. The thin-wall needle helps to ensure sufficient blood collection speed. Inner surface of needle cannula becomes smooth after lubricated by silicone, which can maximally reduce the damage to blood cell when it flows through the cannula, keep the original features of blood specimen and increase the quality of blood specimen. Rubber sleeve on the non-patient needle has excellent performance at multiple puncture and sealing property, which prevents blood leakage even after continuous multiple penetration and achieves multiple collection, easing patient and releasing medical personnel from heavy work.