For rapid and highly reliable identification of RNA from Bovine Viral Diarrhea Virus genotypes 1 and 2 (BVDV-1, BVDV-2) and atypical pestivirus strains (HoBi-like, BVDV-3) in cattle (Availability/distribution: Outside the U.S. and Canada)
Bovine Viral Diarrhea is an economically important infectious disease of cattle.
The virotype BVDV RT-PCR Kit is intended for the detection of BVDV RNA in blood, plasma, serum, milk and ear tissue samples (individual or pooled) from cattle. It contains all the reagents for real-time RT-PCR including a positive and negative control. With this kit, both reverse transcription and PCR are performed in one reaction tube, reducing the risk of contamination. An internal control excludes the possibility of false negative results.
The test kit uses two specific primer/probe combinations: one for pestiviruses yielding FAM™ fluorescence and one for the internal control yielding HEX™ fluorescence.
The high sensitivity of the virotype BVDV RT-PCR Kit permits blood samples (whole blood, plasma, serum) to be tested in pools consisting of up to 50 individual samples, ear tissue samples in pools of up to 25 individual samples, and milk in pools of up to 100 individual samples.
The virotype BVDV RT-PCR Kit is approved in Germany (since 2007), Switzerland (2018), France (2013), Czech Republic (2014) and Belgium (2014) and highly relied upon in BVDV eradication and control programs (e.g., Ireland, Germany, UK).
Product contents - PCR Mix (includes primers, probes and internal control), Enzyme Mix, Positive Control, Negative Control, Handbook