Immunity test kit INOGene

virusHIVretrovirus

INOGene-Human Immunodeficiency Virus (HIV) Quantitative RT-PCR Kit HIV is classified in the family Retroviridae, subfamily Lentivirinae, and genus Lentivirus (1). The struc- ture of HIV follows the typical pattern of the retrovirus family, comprising a singlestranded, positive- sense ribonucleic acid (RNA) genome of about 9.7 kilobases. INOGene-Human Immunodeficiency Virus Quantitative RT-PCR Kit assay is a one-step real time reverse transcription PCR assay in which RNA templates are first reverse-transcribed to generate complementary cDNA strands followed by DNA polymerase-mediated cDNA amplification. INOGene-Human Immunodeficiency Virus Quantitative RT-PCR Kit utilizes external standards to gather quantitative results and includes an internal control, which controls for target isolation and amplification. The target region is situated in gag gene region of HIV-1 genome and is 121-bases long. HIV-1 RNA concentration is noted in International Units/ml (IU/ml).