Solution reagent kit A-R0200000001

blood samplefor clinical chemistryliquid

Product for use in the quantitative determination in vitro of the concentration of the Direct Bilirubin in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical context. For professional use only. CLINICAL SIGNIFICANCE Approximately 80-85 % of the bilirubin produced is derived from the heme moiety of the haemoglobin released from aging erythrocytes in the reticuloendothelial cells. Bilirubin, bound to albumin, is transported into the liver where it is rapidly conjugated with glucuronide to increase its solubility. Then it is excreted into biliary canaliculi, and hydrolyzed in the gastrointestinal tract. Unconjugated bilirubin serum concentration increases in case of overproduction of bilirubin (acute or chronic haemolytic aniemias) and in case of disorders of bilirubin metabolism and transport defects (impaired uptake by liver cells: Gilbert’s syndrome; defects in the conjucation reaction: Crigler-Najjar syndrome). Reduced excretion (hepatocellular damage: hepatitis, cirrhosis, Dubin-Johnson and Rotor syndrome) and obstruction to the flow of bile (most often produced by gallstones or by tumors) induce an important elevation of conjugated bilirubin and in a minor extent an increase of unconjugated bilirubin (conjugated hyperbilirubinemia).