This kit utilizes a new silica membrane technology and reagent formulation to recover 100 bp to 10 kb of DNA fragments from PCR product or enzymatic reactions (such as restriction enzyme digestion, ligation and probe labelling). This kit uses a unique spin column to bind DNA, followed by washing and eluting steps. Each column can bind up to 10 μg of DNA, while maximumly removing impurities such as primers, oligonucleotides, and enzymes.The purified DNA has high purity and concentration, good integrity, and the recovery rate is high. The purified DNA can be directly used for molecular biology experiments such as sequencing, ligation and transformation, labeling, and in vitro transcription.