Enzyme reagent FastSYBR Mixture

DNA polymeraseTaq DNA polymerasefor qPCR

The FastSYBR Mixture is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2×. It contains GoldStar Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I Fluorescent Dye, and Mg2+. The operation is simple and convenient. This product is mainly used for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription. This product contains the fluorescent dye SYBR Green I which can bind with all double-strand DNA, so that the product can be used for the detection of different target sequences without the need for the synthesis of specific labeled probes. The GoldStar Taq DNA Polymerase in the mixture is a chemically-modified, new efficient hot-start enzyme that does not have polymerase activity at room temperature which prevents nonspecific amplification efficiently, and it is activated by incubation at 95°C for 10 minutes. The combination of a unique PCR buffer system and a hot-start enzyme effectively inhibits non-specific PCR amplification and significantly increases the amplification efficiency of PCR.This product is suitable for fluorescent qPCR instruments with low ROX. No ROX as a calibration dye: Roche LightCycler 480, Roche LightCyler 96, Bio-rad iCyler iQ, iQ5, and CFX96. Need Low ROX (30-50mM) as a calibration dye: ABI Prism7500/7500 Fast, QuantStudio® 3 System, QuantStudio® 5 System, QuantStudio® 6 Flex System, QuantStudio® 7 Flex System, ViiA 7 system, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 Need High ROX (300-500mM) as a calibration dye: ABI Prism7000/7300/7700/7900，Eppendorf，ABI Step One/Step One Plus.