This kit is suitable for both Illumina and MGI sequencing platforms. It provides the premixed enzyme modules required for DNA end repair, 5'-end phosphorylation modification, 3'-end A addition and Adaptor ligation in DNA library construction. It can be used with different sequencing platform adapters. Primer kits can prepare DNA into specific DNA libraries for Illumina or MGI sequencing platforms. Using high-fidelity DNA polymerase for library enrichment and unbiased PCR amplification, the coverage area of the sequence is expanded, and high-quality DNA libraries can be prepared. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability of library construction to the greatest extent.