This product is suitable for in vitro qualitative detection of Borrelia burgdorferi nucleic acid in the whole blood of patients, and provides auxiliary means for the diagnosis of Borrelia burgdorferi patients. The test results are only for clinical reference, and the final diagnosis should be comprehensively considered in close combination with other clinical indicators. Lyme disease is caused by infection with Borrelia burgdorferi and is mainly transmitted between animal hosts, between host animals and humans by hard ticks. The pathogen Borrelia burgdorferi can cause human Erythema chronicum migrans, as well as diseases involving multiple systems such as heart, nerve, and joint, etc., and the clinical manifestations are diverse. According to the developing of diseases course, it can be divided into early localized infection, intermediate disseminated infection and late persistent infection, which are serious harm to the health of the population. Therefore, in the clinical diagnosis of Borrelia burgdorferi, it is of great significance to establish a simple, specific and rapid method for etiological diagnosis of Borrelia burgdorferi.