Genetic mutation test kit HWTS-TM008B

for cancersfor BRAF mutationsFFPE tissues

This test kit is used to qualitatively detect the BRAF gene V600E mutation in paraffin-embedded tissue samples of human melanoma, colorectal cancer, thyroid cancer and lung cancer in vitro. The protein encoded by the BRAF gene is a serine/threonine specific protein kinase that exists in the cytoplasm. It is an important signal regulator in the RAS/RAF/MEK/ERK/MAPK pathway, and is involved in the regulation of various biological activities in cells, such as cell growth, differentiation and apoptosis [1]. More than 30 types of BRAF mutations have been found, of which about 90% are located in exon 15, where V600E mutation is considered to be the most common mutation, that is, thymine(T) at position 1799 in exon 15 is mutated to adenine (A), resulting in the replacement of valine (V) at position 600 by glutamic acid (E) in the protein product [2-3]. This V600E mutation leads to increased kinase activity of BRAF, which in turn continuously activates the downstream MAPK signaling pathway, causing hyperproliferation and malignant transformation of tumor cells.