Infectious disease test kit HWTS-FG005A

for urinary diseasesCandida albicansCandida tropicalis

This kit is intended for the in vitro qualitative detection of the nucleic acid of Candida tropicalis in genitourinary tract samples or clinical sputum samples. The test result of this kit is not the only indicator for the diagnosis of Candida albicans infection in patients. It must be combined with the patient’s clinical characteristics, imaging examinations, and other laboratory test data to correctly identify whether the fungus is infection or colonization and to formulate a reasonable treatment protocol to make the anti-fungal treatment be safe and effective. Sample processing 1. Genitourinary tract swab 1.1 Add 1-3mL of sterile normal saline to the sterile tube containing the swab specimen, and shake and mix thoroughly. Squeeze the cotton swab and discard it. 1.2 Pipette 1mL of liquid to a 1.5mL RNase-free & DNase-free centrifuge tube (if there are too many secretions, take 0.2mL only), centrifuge at 12000rpm for 3min, discard the supernatant and keep the precipitate. 1.3 Add 180μL of lysozyme buffer (dilute the lysozyme to 20mg/mL with lysozyme diluent) to the precipitate, shake and mix, and treat at 37°C for 30min. 1.4 Take a 1.5mL RNase-free & DNase-free centrifuge tube, and add 200μL of positive control and negative control in sequence. Add 3μL of internal reference control to the sample to be tested, the positive control, and the negative control in sequence, and use the recommended nucleic acid extraction reagents for subsequent sample DNA extraction. Please strictly follow the instructions for use for specific steps. Use DNase and RNase-free water for elution. The recommended elution volume is 100μL.