This kit is suitable for the qualitative detection of Mycobacterium tuberculosis DNA in human sputum samples in vitro, as well as the homozygous mutation in the 507-533 amino acid codon region of the rpoB gene that causes Mycobacterium tuberculosis rifampicin resistance.
Epidemiology
Mycobacterium tuberculosis , shortly as Tubercle bacillus, TB, is the pathogenic bacterium that causes tuberculosis. Currently, the commonly used first-line anti-tuberculosis drugs include isoniazid, rifampicin and hexambutol, etc. The second-line anti-tuberculosis drugs include fluoroquinolones, amikacin and kanamycin, etc. The new developed drugs are linezolid, bedaquiline and delamani, etc. However, due to the incorrect use of anti-tuberculosis drugs and the characteristics of the cell wall structure of mycobacterium tuberculosis, mycobacterium tuberculosis develops drug resistance to anti-tuberculosis drugs, which brings serious challenges to the prevention and treatment of tuberculosis.
Rifampicin has been widely used in the treatment of pulmonary tuberculosis patients since the late 1970s, and has a significant effect. It has been the first choice to shorten the chemotherapy of pulmonary tuberculosis patients.