This kit is used for the qualitative detection of staphylococcus aureus and methicillin-resistant staphylococcus aureus nucleic acids in human sputum samples, skin and soft tissue infection samples, and whole blood samples in vitro. Sputum sample testing is used in medical institutions to prevent and control MRSA nosocomial infection in hospitalized patients, including intensive care patients, surgical patients and long-term care patients; skin and soft tissue infection sample testing is used in combination with other laboratory tests such as microbial culture to assist diagnose skin and soft tissue infections of MRSA/SA; whole blood sample testing is used for auxiliary diagnosis of MRSA/SA infection in patients who need blood culture detection.