Genetic mutation test kit HWTS-TM014

for KRAS mutationsFFPE tissuesfor RT-PCR

This kit is intended for in vitro qualitative detection of 8 mutations in codons 12 and 13 of K-ras gene in extracted DNA from human paraffin-embedded pathological sections. Point mutations in the KRAS gene have been found in a number of human tumor types, about 17%~ 25% mutation rate in tumor, 15%~ 30% mutation rate in lung cancer patients, 20%~ 50% mutation rate in colorectal cancer patients. Because the P21 protein encoded by the K-ras gene is located downstream of the EGFR signaling pathway, after the K-ras gene mutation, the downstream signaling pathway is always activated and is not affected by the upstream targeted drugs on EGFR, resulting in continuous malignant proliferation of cells. Mutations in the K-ras gene generally confer resistance to EGFR tyrosine kinase inhibitors in lung cancer patients and resistance to anti-EGFR antibody drugs in colorectal cancer patients [1, 2, 3]. In 2008, the National Comprehensive Cancer Network (NCCN) issued a clinical practice guideline for colorectal cancer, which pointed out that the mutation sites that cause K-ras to be activated are mainly located in codons 12 and 13 of exon 2, and recommended that all patients with advanced metastatic colorectal cancer can be tested for K-ras mutation before treatment[4].