This kit is used for genotyping detection of hepatitis C virus (HCV) subtypes 1b, 2a, 3a, 3b and 6a in clinical serum/plasma samples of hepatitis C virus (HCV). It aids in diagnosis and treatment of HCV patients.
Epidemiology
Hepatitis C virus (HCV) belongs to the family flaviviridae, and its genome is a single positive strand RNA, which is easily mutated. The virus exists in the hepatocytes, serum leukocytes and plasma of infected persons. HCV genes are susceptible to mutation and can be divided into at least 6 genotypes and multiple subtypes. Different HCV genotypes use different DAAs treatment regimens and courses of treatment. Therefore, before patients are treated with DAA antiviral therapy, the HCV genotype must be detected, and even for patients with type 1, it is necessary to distinguish whether it is type 1a or type 1b.
Technical Parameters
Storage - ≤-18℃ In dark
Shelf-life - 9 months
Specimen Type - Serum, Plasma
Ct - ≤36
CV - ≤5.0%
LoD - 200 IU/mL
Specificity - Use this kit to detect other virus or bacterial samples such as: human cytomegalovirus, Epstein-Barr virus, human immunodeficiency virus, hepatitis B virus, hepatitis A virus, syphilis, human herpes virus type 6, herpes simplex virus type 1, simplex Herpes virus type 2, influenza A virus, Propionibacterium acnes, Staphylococcus aureus, Candida albicans, etc. The results are all negative.
Applicable Instruments
It can match the mainstream fluorescent PCR instruments on the market.
ABI 7500 Real-Time PCR Systems
ABI 7500 Fast Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems