Y Chromosome DNA Microdeletions qPCR Detection Ki
Ychromosome microdeletion is a disease lead by gene problem. It will affectsperm production when azoospermia exists on the Y chromosome. However, the genelocus is too small to be detected by the conventional methods.
This kit canbe used for the diagnosis of Y-chromosomal microdeletionsof the AZFa (sY84 and sY86), AZFb (sY127 and sY134), and AZFc (sY254 and sY255)region on azoospermic and oligozoospermic men.
32 Reactions/Kit
Applicable Instrument
ABI 7500 Real-Time PCR System
Samples
Human EDTA anticoagulant whole blood, human sodium citrateanticoagulant whole blood, or human oral exfoliated cell.
Pathogens
Assay for the detection of Y chromosome microdeletion
Methods
Multiplex qPCR method, Taqman fluorescent probe technology
Detection
Y-chromosomal microdeletions of the AZFa (sY84 and sY85), AZFb (sY127 and sY134) and AZFc (sY254 and sY255)
Indications
ICSi therapy auxiliary diagnosis male patients with oligospermia and azoospermia
Male infectility with normal sperm density
Male infertility with cryptorchidism and varicocele
Unexplained habitual abortion of patients' wife sperm library screening
Assay
Two PCR reaction solutions
Results
Automatic data reports
Efficiency
Real-time
High-accuracy
Indexes
Coincidence rate:100%
Variation coefficient: less than 5%
No cross reactions