Flu test kit

influenza Ainfluenza Bchromatographic immunoassay

Influenza A/B Antigen Detection Kit(Colloidal Gold Method) is a lateral flow chromatographic immunoassay. The test strip consists of: 1) a burgundy colored conjugate pad containing anti-Influenza A and B antibody onjugated with colloidal gold (antibody conjugates) 2) a nitrocellulose membrane strip containing two test lines (T1 and T2 lines) and a control line (C line). The T1 line is pre-coated with anti-Influenza A antibody, the T2 line is pre-coated with anti-Influenza B antibody, and the C line is pre-coated with a control line antibody. The Influenza antigen is firstly extracted from the specimen with extraction buffer. The antigen extracts contact the test strip and then migrate by capillary action across the test strip. Influenza A antigen, if presents in the extract, will bind to the antibody conjugates. The immunocomplex is then captured on the membrane by the pre-coated anti-Influenza A antibodies, forming a burgundy colored T1 line, indicating an Influenza A positive test result. Influenza B antigen, if present in the extract, will bind to the antibody conjugates. The immunocomplex is then captured on the membrane by the pre-coated anti-Influenza B antibodies, forming a burgundy colored T2 line, indicating Influenza B positive test result. The test contains an internal control (C line) which should exhibit a burgundy colored line of the of the control antibodies regardless of color development on any of the test lines. If the C line does not develop, the test result is invalid and the specimen must be retested with another device.