Solution reagent kit DepleteX®

for RNA boost

This product is a new and improved replacement for CRISPRclean Single Cell RNA Boost Kit (KIT1018). DepleteX™️ removes abundant and uninformative fragments before sequencing, redistributing 50% sequencing clusters to informative reads that normally would be filtered out prior to secondary analysis. This kit leverages Cas9 depletion with an optimized guide set to remove ribosomal and mitochondrial mRNA, non-transcriptomic reads, and highly expressed non-variable genes. This new kit includes a separate tube for non-variable gene depletion content that can be included or excluded as needed. DepleteX Single Cell RNA Boost Kit gives you the ability to cut through the noise and maximize usable data in your single cell RNA sequencing. Highlights ~50% boost in reads useful for secondary analysis Validated for both short and long read sequencing Depletes ribosomal, mitochondrial, non-transcriptomic reads, and non-variable genes (optional) from single cell libraries Simple 3 step protocol Specification Samples per kit - 24 Assay time - 2 hours Hands-on-time - 45 minutes Input - Uses one of four Chromium cDNA aliquots per prep Method - Single cell RNA-Seq Designed to deplete - ribosomal, mitochondrial, non-transcriptomic reads, and non-variable genes (optional) Jumpcode validated - 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3.1, Visium Spatial Gene Expression libraries, PacBio Iso-Seq SMRTbell Express Template Prep Kit 2.0, BD Rhapsody Customer validated - 10x Genomics Chromium 5' and multiome, Parse Biosciences Evercode Whole Transcriptome