Solution reagent kit KIT1020

for RNA depletion

Our DepleteX High Expressing RNA Depletion kit removes the highest expressing transcripts in-vitro ahead of sequencing, redistributing ~70%* sequencing reads to unique biologically relevant transcripts—allowing you to maximize detection of low expressing transcripts or rare isoforms. Boost detection sensitivity in fibroblasts and whole blood Resequencing high expressing transcripts adds little biologically relevant information. Unfortunately, these transcripts outcompete low expressing transcripts for sequencing coverage. This lack of coverage, coupled with significant noise from high expressing transcripts, limits detection of the low expressing genes, rare variants and unique fusion events that may be disease drivers. DepleteX leverages Cas9 and a guide RNA set designed to remove 70% of the highest expressing transcripts from poly-A selected RNA-Seq libraries derived from fibroblasts and whole blood. This guide set was designed to maximize the re-assignment of total sequencing reads to increase sequencing coverage of rare, low expressing transcripts and unique fusion events. Highlights Deplete >4,000 highly expressed transcripts in whole blood and fibroblasts Assign ~70%* more reads to low-expressing biologically relevant transcripts Maximize variant and isoform detection with less sequencing Increased detection of lower expressing transcripts Integrate easily into your workflow with a simple and flexible protocol About Kit includes guide RNA and depletion reagents for post-library depletion. Specification Samples per kit - 24 Tissues evaluated in design - Fibroblast, whole blood Depletion content - ~4,450 high expressing transcripts