Solution reagent kit DepleteX™

for RNA depletion

DepleteX Globin Depletion Kit removes four highly expressed globin transcripts in-vitro ahead of sequencing, redistributing sequencing clusters to unique biologically relevant transcripts —allowing you to maximize UMI and gene sensitivity. Beyond ribodepletion, RNA samples typically have a small number of highly expressed, well characterized transcripts, and resequencing these transcripts adds little biologically relevant information. Unfortunately, these highly expressed transcripts outcompete low expressing transcripts for sequencing coverage. This lack of sequencing coverage coupled with significant noise from high expressing transcripts limits detection of low expressing genes, rare variants and unique fusion events that may be disease drivers. Highlights Simple unbiased depletion of human globin from RNA-Seq libraries Achieve >99% depletion of contaminating globin Quick one hour depletion incubation About Kit designed to deplete four human globin genes (HBA1, HBA2, HBB, HBD) Specification Samples per kit - 24 Tissues evaluated in design - Blood Designed to deplete - HBA1, HBA2, HBB, and HBD CRISPRclean product compatibility - Single cell and ribodepletion