DepleteX RNA depletion panel removes the highest expressing insulin transcripts in-vitro ahead of sequencing, redistributing sequencing clusters to unique biologically relevant transcripts —allowing you to maximize UMI and gene sensitivity.
Beyond ribodepletion, RNA samples typically have a small number of highly expressed, well characterized transcripts, and resequencing these transcripts adds little biologically relevant information. Unfortunately, these highly expressed transcripts outcompete low expressing transcripts for sequencing coverage. This lack of sequencing coverage coupled with significant noise from high expressing transcripts limits detection of low expressing genes, rare variants and unique fusion events that may be disease drivers.
Highlights
Insulin panel designed to deplete overexpressing transcripts in pancreas samples
Re-assign sequencing reads to lower expressing transcripts for increased coverage
Maximize variant and isoform detection with less sequencing
About
Kit contains tissue-specific guide RNA and depletion reagents for post-library depletion
Specification
Samples per kit - 24
Tissues evaluated in design - Pancreas
Designed to deplete - 2 insulin genes