Solution reagent kit DepleteX™
for RNA depletionfor insulin

Solution reagent kit - DepleteX™ - Jumpcode - for RNA depletion / for insulin
Solution reagent kit - DepleteX™ - Jumpcode - for RNA depletion / for insulin
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Characteristics

Type
solution
Applications
for RNA depletion
Tested parameter
for insulin

Description

DepleteX RNA depletion panel removes the highest expressing insulin transcripts in-vitro ahead of sequencing, redistributing sequencing clusters to unique biologically relevant transcripts —allowing you to maximize UMI and gene sensitivity. Beyond ribodepletion, RNA samples typically have a small number of highly expressed, well characterized transcripts, and resequencing these transcripts adds little biologically relevant information. Unfortunately, these highly expressed transcripts outcompete low expressing transcripts for sequencing coverage. This lack of sequencing coverage coupled with significant noise from high expressing transcripts limits detection of low expressing genes, rare variants and unique fusion events that may be disease drivers. Highlights Insulin panel designed to deplete overexpressing transcripts in pancreas samples Re-assign sequencing reads to lower expressing transcripts for increased coverage​ Maximize variant and isoform detection with less sequencing About Kit contains tissue-specific guide RNA and depletion reagents for post-library depletion Specification Samples per kit - 24 Tissues evaluated in design - Pancreas Designed to deplete - 2 insulin genes
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