CPV and CCVin dog feces were quantitatively detected by fluorescence immunochromatography. Basic principle: there are T and C lines on the nitric acid fiber membrane,T1 and T2 lines are respectively coated with antibodies a and b that specifically recognize CPV and CCV antigens. Combination mat is sprayed with antibodies c and d labeled with another fluorescent nano material that can specifically recognize CPV and CCV,the CPV / CCV in the sample first combined with the nanomaterial markers antibodies c and d forming complexes, and then chromatography upward, the complexs combined with the corresponding T1 and T2 lines, when the excite light irradiate, nanometer material emission fluorescence signal, and the signal strength andCPV and CCV concentration in the samples into positive correlation.
Detailed
【Detection purpose】 Canine Parvovirus (CPV) belongs to the parvovirus genus of Parvoviridae, which can cause severe infectious diseases in dogs. Generally, there are two clinical manifestations: hemorrhagic enteritis and myocarditis. Both have the characteristics of high mortality, strong infectivity and short course of disease, especially in puppies. Canine coronavirus (CCV) belongs to the genus coronavirus of coronavirus family. It is a highly harmful canine infectious disease. The general clinical manifestations are: gastroenteritis symptoms, specifically vomiting, diarrhea and anorexia. CPV and CCV mixed infection, so reliable and effective detection plays a positive guiding role in prevention, diagnosis and treatment.