Solution reagent kit TMB PLUS2®

laboratoryliquidhydrogen peroxide

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride). TMB PLUS2® characteristics are: Medium sensitivity Very low background Aqueous based – no organic solvents (REACH Annex XVII compliant) Low lot-to-lot variation Suitable for use in electrochemical assays Specifications Available sizes: 100 mL, 500 mL, 1 L, bulk Format: Ready-to-use (RTU) Stability: 4 years Development time: 5-30 minutes Stop solution: 0.2 M H₂SO₄