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Solution reagent kit TMB X-tnd®
laboratoryliquidhydrogen peroxide

solution reagent kit
solution reagent kit
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Characteristics

Type
solution
Applications
laboratory
Format
liquid
Tested parameter
hydrogen peroxide
Method
enzymatic, chromogenic
Storage temperature

Min.: 2 °C
(36 °F)

Max.: 8 °C
(46 °F)

Description

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm. TMB X-tnd is developed to support assays, which require low detection limits while supporting OD readings of highly positive samples – with no precipitation of the TMB substrate, at up to 5.8 mAbs within 60 minutes. TMB X-tnd® characteristics are: Best dynamic range No precipitation Aqueous based – no organic solvents (REACH Annex XVII compliant) Low lot-to-lot variation Suitable for use in electrochemical assays Specifications Available sizes 100 mL, 500 mL, 1 L, bulk Format: Ready-to-use (RTU) Stability: 4 years Development time: 5-30 minutes Stop solution: 0.35 M H₂SO₄
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