pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to pnitrophenol and inorganic phosphate. Liberated pnitrophenol is seen as a clear yellow color that is measured at 405/620 nm.
pNPP ONE liquid ready-to-use substrate is active and stable.
Specifications
Available sizes:
100 mL, 500 mL, 1 L, bulk
Format:
Ready-to-use (RTU)
Stability:
3 years
Development time:
5-30 minutes
Stop Solution:
0,1 M NaOH