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Solution reagent kit pNPP
buffer solutionlaboratoryliquid

solution reagent kit
solution reagent kit
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Characteristics

Type
solution, buffer solution
Applications
laboratory
Format
liquid
Tested parameter
phosphate, alkaline phosphatase
Method
chromogenic
Storage temperature

Min.: 2 °C
(36 °F)

Max.: 8 °C
(46 °F)

Description

pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to p-nitrophenol and inorganic phosphate. Liberated p-nitrophenol is seen as a clear yellow color that is measured at 405/620 nm. pNPP liquid ready-to-use substrate is highly active compared to pNPP ONE . The substrate gives superior results due to its sensitivity, allowing very low detection limits. Specifications Available sizes: 100 mL, 500 mL, 1 L, bulk Format: Ready-to-use (RTU) Stability: 2 years Development time: 5-30 minutes Stop Solution: 0,1 M NaOH
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