pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to p-nitrophenol and inorganic phosphate. Liberated p-nitrophenol is seen as a clear yellow color that is measured at 405/620 nm. pNPP liquid ready-to-use substrate is highly active compared to pNPP ONE . The substrate gives superior results due to its sensitivity, allowing very low detection limits.
Specifications
Available sizes:
100 mL, 500 mL, 1 L, bulk
Format:
Ready-to-use (RTU)
Stability:
2 years
Development time:
5-30 minutes
Stop Solution:
0,1 M NaOH