Cell culture bioreactor SB50-X

for scientific researchsingle-usewith orbital shaker

The SB50-X is an orbital shaken bioreactor for the cultivation of human, mammalian and plant cells in a single-use bag. A working volume from 15 litres up to 50 litres for the SB50-X ensures easy scale-up from microtiter plates to shaking flasks and all the way up to 200 litres. The SB50-X can be used in research, process development or production. The SB50-X was developed and tested in collaboration with ExcellGene SA (Monthey, Switzerland) and the Swiss Federal Institutes of Technology EPFL (Lausanne, Switzerland). Performance characteristics Large capacity (50L working volume) single use bioreactor system Cultivation of human, mammalian and plant cells Fast, simple set up Easy scale-up Online measurement of pH and DO Bag Single-use bag for fast and simple set up The cylindrical vessel accepts a single-use bag. This bag requires no additional mixing device, enables quick set up times and eliminates elaborate cleaning and sterilising procedures. Various ports are incorporated to allow feeding, inoculation, harvesting and sampling. The bag has built in optical sensors for pH and DO. Orbital motion The orbital motion of the SB50-X ensures efficient liquid mixing. It allows high oxygen transfer rates with low shear forces. Mechanical stress on the cells is reduced thanks to bubble-free surface gassing with almost no frothing. This motion is particularly suitable for microcarrier cultivation. The SB50-X uses the unique Kuhner direct drive which provides accurate control of the orbital speed.