Real time PCR LB-11RTPCR is a microprocessor controlled dual channeled unit. It is characterized with a photomultiplier tube as a detector for amplification. Quantitative estimation of targeted DNA molecule is carried out during conventional PCR.
Features
Advance temperature control
Precise thermoelectric system
Momentary power failure protection
Intelligent auxiliary baseline selection
Non-maintenance optical system
RS232c system interface
Applications
Used for quantitative and semi quantitative analysis for real time PCR in diagnosis of clinical diseases, animal diseases, food safety, research purpose, etc.
Specifications
Testing range - 101 DNA/RNA copy/mL ~ 1010 DNA/RNA copy/mL
Heating rate - 3 °C / sec
Cooling rate - 2 °C / sec
Profiles - 1 ~ 9
Cycles - 1 ~ 99
Hold time - 1 sec ~ 9999 sec
Excitation wavelength - Channel 1: 470 nm ~ 505 nm
Channel 2: 525 nm ~ 545 nm
Emission wavelength - Channel 1: 523 nm ~ 543 nm
Channel 2: 550 nm ~ 615 nm
Consumables - Thick walled 0.2 mL PCR tubes
8-strip tubes
Reaction volume - 10 µL ~ 100 µL
Correlation coefficient of nucleic acid precision - R ≥ 0.0997
Reproducibility - CV ≤ 2.1 %
Temperature accuracy - ± 0.1 °C
Temperature uniformity - ± 0.3 °C
Ambient temperature - 5 °C ~ 40 °C
Relative humidity - ≤ 80 %