Solution reagent kit SLAMseq

NGSfor single cell RNA sequencingRNA Synthesis

Analyze transcriptome-wide kinetics of RNA synthesis and turnover Measure nascent RNA expression and transcript stability Enhance the temporal resolution of differential expression Identify primary and secondary transcriptional targets No pull-down or biochemical isolation required Use in combination with QuantSeq 3’ mRNA-Seq for cost-effective, high-throughput metabolic sequencing SLAMdunk - automated and user-friendly SLAMseq-QuantSeq data analysis pipeline on the BlueBee ® genomics analysis platform SLAMseq is a high-sensitivity method for time-resolved measurement of newly synthesized and existing RNA in cultured cells. SLAMseq enables resolution of RNA synthesis and degradation kinetics. Lexogen offers a family of kits based on the new SLAMseq method: Thiol (SH)-Linked Alkylation for the Metabolic sequencing of RNA. SLAMseq enables the identification and quantification of newly synthesized (nascent) and existing RNA from the same sample in parallel, without the need for biochemical isolation. SLAMseq can be readily applied to living cell experiments. Combined with QuantSeq 3’ mRNA-Seq library preparation, SLAMseq provides a complete user friendly and high-throughput solution for analyzing transcriptome-wide RNA synthesis and turnover kinetics. The SLAMseq kits portfolio includes Explorer and Kinetics Modules, which cover the entire metabolic RNA labeling experiment, from optimizing labeling conditions, to labeling of nascent or existing RNA and alkylation for downstream NGS library preparation.