Solution reagent kit QuantSeq

NGSfor RNA library preparation

Cost-saving and streamlined globin mRNA depletion during QuantSeq library preparation 3’ UTR analysis and study of alternative polyadenylation Down to 10 ng total RNA input (incl. FFPE samples) Free data analysis pipeline on the BlueBee® genomics analysis platform Cost-efficient sequencing of up to 9,216 samples/lane (single-read or paired-end) The QuantSeq REV Kit is a library preparation protocol designed to generate Illumina compatible libraries of sequences at the 3’ end of the polyadenylated RNA. With QuantSeq Reverse (REV) it is possible to exactly pinpoint the 3’ end in Read 1, hence to study the 3’ UTR and alternative polyadenylation. With the help of the Custom Sequencing Primer (CSP Version 2, included in the kit) the first nucleotide of your NGS read corresponds the very last nucleotide of the mRNA. The reads generated during Read 1 reflect the cDNA sequence. Analysis of Low Input and Low Quality Samples The required input amount of total RNA is as low as 10 ng. QuantSeq is suitable to reproducibly generate libraries from low quality RNA, including FFPE samples. Rapid Turnaround QuantSeq’s simple workflow allows generating ready-to sequence NGS libraries within only 4.5 hours, including less than 2 hours hands-on time. Deplete globin mRNAs during QuantSeq Library Prep New! Globin Block Modules for QuantSeq enable the generation of globin-depleted, ready-to-sequence 3’ mRNA-Seq libraries from as little as 50 ng of total RNA from whole blood.