Solution reagent kit QuantSeq-Flex

NGSfor RNA library preparation

Flexible kit for targeted sequencing and molecular barcoding Identification of known and unknown fusion transcripts Use of custom-designed gene-specific primers for first and/or second strand synthesis Cost-efficient and flexible alternative to pre-designed sequencing panels Sequencing of up to 9,126 samples/lane The QuantSeq-Flex Kit is a library preparation protocol designed to make Illumina compatible libraries from any RNA sample using custom primers. It is open for development by advanced RNA-Seq users based on their custom needs. The kit is based on the QuantSeq FWD Kit for Illumina (Cat. No. 015) strategy, with compatible reagents and Read 1 linker sequence introduced by the second strand synthesis primer. Hence the reads generated during the NGS run directly correspond to the RNA sequence. QuantSeq-Flex Kit provides modules for Reverse Transcription (RT) and SSS, allowing users to choose their own primers for either one of these steps or both of them. This offers maximum flexibility in the choice of targets and input material. With this highly flexible QuantSeq kit the following types of libraries can be generated: 1.) OligodT primed in reverse transcription, random primed in second strand synthesis (QuantSeq 3’ mRNA-Seq) 2.) OligodT primed in reverse transcription, target-specifically primed in second strand synthesis (targeted 3’ mRNA-Seq) 3.) Target-specifically primed in reverse transcription, random primed in second strand synthesis (targeted RNA-Seq, allows for identification of novel fusions) 4.) Target-specifically primed in reverse transcription, target-specifically primed in second strand synthesis (targeted RNA-Seq, known targets detectable only)