Solution reagent kit TraPR

for RNA extractionNGS

Isolate only fully functional, physiologically relevant silencing sRNAs Save time by using an easy single-column 15-minute workflow Extract high-quality sRNA even from challenging samples Screen for novel sRNAs in uncharacterized samples Simplify downstream analysis and save sequencing cost TraPR Small RNA Isolation Kit TraPR (Trans-kingdom, rapid, affordable Purification of RISCs) presents a gel- and bias-free, column-based method for isolation of functional small RNAs from RNA-induced silencing complexes (RISCs) of all organisms. Within 15 minutes, TraPR enables purification of RISC fractions even from challenging or inconsistent samples, cell types, tissues, and bio-fluids. The TraPR Small RNA Isolation Kit generates high-quality sRNA preparations suitable for Next Generation Sequencing (NGS) applications and thus provides a highly reproducible, time-saving method that outperforms all current gold-standard procedures for sRNA profiling. TraPR Eliminates the Need for Gel Extraction of sRNAs Typically, sRNA-Seq libraries from total RNA contain only a minor fraction of reads corresponding to functional sRNAs (Fig. 1A). Thus, size selection methods such as gel extraction are commonly applied to increase the share of sRNA-mapping reads (Fig. 1B). TraPR replaces these lengthy and error-prone gel extractions with quick and easy column purifications (Fig. 1C).