Solution reagent kit CORALL

NGSfor RNA library preparation

Complete coverage of transcripts from start to end Ready-to-sequence libraries within 4.5 hours Unique Molecular Identifiers (UMIs) seamlessly included Excellent protocol-inherent strandedness (>99 %) 1 ng to 1 µg of total RNA input Easy all-in-one protocol The CORALL Total RNA-Seq Library Prep Kit enables fast and cost-efficient generation of UMI labelled, stranded libraries for whole transcriptome analyses using Illumina® NGS platforms. Gene Detection CORALL delivers excellent gene discovery rates matching conventional competitor kits (Fig. 2A). 95% of genes are commonly detected between CORALL and each of the two competitors (at >10 counts per million (CPM), Fig. 2B). This high level of library complexity ensures faithful representation of the transcriptome, enabling sensitive expression profiling Superior End-to-End Coverage CORALL’s comprehensive coverage delivers improved transcript start and end site representation. Read coverage was analyzed using the ERCC spike-in controls, which feature precise, known transcription start and end sites (TSS and TES, respectively). CORALL reads map more accurately to the exact ERCC TSS (Fig. 3A) than competitor libraries, which fail to cover the true start sites. Additionally, CORALL provides elevated coverage at TES