Solution reagent kit

NGSfor RNA library preparation

mRNA-Seq including poly(A) selection Wide range of total RNA input (1 ng to 1 μg) Easy all-in-one workflow From poly(A) selection to ready-to-sequence libraries in 5.5 hours Unique Molecular Identifiers (UMIs) included 12 nt Unique Dual Indices (UDIs) included The CORALL mRNA-Seq Library Prep Kit enables fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries for whole transcriptome poly(A) RNA analyses using Illumina® NGS platforms. Superior End-to-End Coverage CORALL’s comprehensive coverage delivers improved transcript start and end site representation. Read coverage was analyzed using the ERCC spike-in controls, which feature precise, known transcription start and end sites (TSS and TES, respectively). CORALL reads map more accurately to the exact ERCC TSS (Fig. 3A) than competitor libraries, which fail to cover the true start sites. Additionally, CORALL provides elevated coverage at TES (Fig. 3B). Data Analysis A Data Analysis Pipeline is now available on the BlueBee® Genomics Platform. The provided pipeline enables kit users to perform read quality control, mapping, Unique Molecular Identifier (UMI) deduplication, and transcript quantification. For using your activation code register an account with BlueBee (https://lexogen.bluebee.com/portal) and upload your data (fastq.gz files). NOTE! BlueBee Data Analysis is available for a range of species, please refer to FAQ 7. Reference genomes for new species can be added upon request. Please note this will incur a fee. See CORALL Data Analysis, for further details.