Solution reagent kit Small RNA-Seq

sample preparationNGSfor nucleic acids

Gel-free user-friendly workflow Multiplexing of up to 96 samples Ready-to-sequence libraries in less than 5 hours Wide input range from 50 pg to 1,000 ng RNA Optimized for low RNA content samples such as plasma, serum, and urine Description Small RNA-Seq Library Prep Kit for Illumina The Small RNA-Seq Library Prep Kit provides a protocol for generating small RNA libraries for Illumina sequencing directly from total RNA or enriched small RNA. Multiplexing of up to 96 Samples Multiplexing of up to 96 samples is possible with complimentary i7 indexes provided in the kit. This allows you to pool more samples per sequencing lane and perform cost-efficient experiments on the platforms of different scale – from bench top to high throughput instruments. Gel-free User-Friendly and Fast Protocol Lexogen’s Small RNA-Seq kit offers a time saving protocol that can be completed within 5 hours, and requires just about 1 hour of your hands-on time. The final library does not need to go through gel purification. Quick and convenient magnetic bead-based purification can optionally be performed to remove adapters dimers for some demanding RNA inputs. Optimized for RNA from Challenging Sources The high sensitivity of the protocol makes it very well suited for challenging, low content RNA sources, such as liquid biopsies (plasma, serum, and urine), including exosomes. The protocol has also been tested on RNA from cells and tissues. The kit can be used for inputs from 100 ng – 1,000 ng of cellular total RNA or 50 pg – 1,000 ng enriched small RNA including plasma, serum, and urine.