Solution reagent kit QuantSeq

sample preparationNGSfor nucleic acids

Cost-efficient genome wide analysis of gene expression Saving sequencing depth by generating only one fragment per transcript Fast and simple all-in-one protocol: less than 4.5 hours Suitable for low input (1 ng total RNA) and FFPE samples Free data analysis pipeline for non-bioinformaticians UMIs for detection of PCR duplicates available NEW! Bundles with up to 384 Unique Dual Indices (UDIs) The QuantSeq FWD Kit is a library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA. QuantSeq FWD contains the Illumina Read 1 linker sequence in the second strand synthesis primer, hence NGS reads are generated towards the poly(A) tail, directly reflecting the mRNA sequence (see workflow). This version is the recommended standard for gene expression analysis. Lexogen furthermore provides a high-throughput version with optional dual indexing (i5 and i7 indices) allowing up to 9,216 samples to be multiplexed in one lane. Mapping of Transcript End Sites By using longer reads QuantSeq FWD allows to exactly pinpoint the 3’ end of poly(A) RNA (see Fig. 3) and therefore obtain accurate information about the 3’ UTR. Analysis of Low Input and Low Quality Samples The recommended input amount of total RNA is as low as 1 ng. QuantSeq is suitable to reproducibly generate libraries from low quality RNA, including FFPE samples. See Fig.1 and 2 for a comparison of two different RNA qualities (FFPE and fresh frozen cryo-block) of the same sample.