Acetaldehyde assay kit LS-K276

for researchNADacetic acid

ACETALDEHYDE (CH3CHO) is one of the most widely occurring aldehydes in nature and is commonly used in industry. Acetaldehyde, a metabolic byproduct of ethanol in the liver, is toxic to the human body and is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing often referred to as "Asian flush syndrome." Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine. BioAssay Systems' colorimetric acetaldehyde assay kit is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the formed NADH reduces a formazan reagent. The intensity of the product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample. Type Detection/Quantition Usage Acetaldehyde determination in biological and food/beverage samples by a colorimetric (565nm) method. Applications Spectrophotometry (visible) Equipment Microplate spectrophotometer (visible) Supplied Components The following components are supplied with this product. Assay Buffer Enzyme A NAD/MTT Solution Enzyme B Standard (See Datasheet for specific volumes supplied) Conditions Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.