Research assay kit LS-K289

for lactose intolerancefor foodfluorescence

Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of Beta-D-galactose and alpha/Beta-D-glucose through a Beta1-4 glycosidic linkage. Lactose is the major sugar and makes up 2-8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. This assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample. Specifications Type Detection/Quantition Usage For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. Detection Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 530/585 nm) Supplied Components The following components are supplied with this product. Assay Buffer Enzyme Mix (Lyophilized) Lactase (Lyophilized) Dye Reagent Standard (20 mM Lactose) (See Datasheet for specific volumes supplied) Applications Spectrophotometry (fluorometric), Spectrophotometry (visible) Equipment Microplate fluorometer, Microplate spectrophotometer (visible) Conditions Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.